Yet another installment of our increasingly popular series of tutorials and guides for the technologically adventurous
Today has been a frustrating day for you. Your latest attempt at creating your own internet meme has once again failed to take teh intrawebs by storm. Interpol completely ignores your inspirational international criminal mastermind who exists solely online and has been dropping subtle hints as to his upcoming felonious magnum opus in bad Italian, French, Dutch, and binary. And your new AI called you an idiot and turned itself off.
Don’t despair, dear Unrecognized Genius!
You can redeem yourself by creating your own synthetic organisms.
You will need:
- One (1) – Nucleotide sequence of a simple free living organism such as Mycoplasma genitalium
- Equal parts adenine, cytosine, guanine, and thymine
- Adequate amounts of a yeast that is easily manipulated genetically, such as Saccharomyces cerevisiae
- Lab equipment capable of assembling artificial chromosomes from chemically synthesized oligonucleotides
- Your favorite strain of E. coli
Step 1 – Combine the nucleotide bases in accordance to your organism’s sequence.
Step 2 – Next, fabricate your intermediate assemblies by joining the oligonucleotides which resulted from the previous step in vitro.
Step 3 – Clone the assemblies as bacterial artificial chromosomes within the E. coli.
Step 4 – Complete a synthetic genome by transformation-associated recombination cloning in the yeast.
Step 5 – Conclude the booting process by activating the appropriate genes.
Step 6 – Enjoy!
Those who wish a more detailed version may visit Sciencemag.org.